Week of 11/25/19 - Happy Thanksgiving!


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Week of 11/11/19

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11/12 Planned what I needed to have ready in order to perform PEMS on the final batch of inoculated flasks. 11/14 Inoculated flasks from the 10/26 plates and placed them in the incubator. 11/15 Prepped the plates needed to show the inactivation of bacteria for the PEMS process. I also did some research on various Deinococcus  species. 11/16 Busy day. Flasks were taken out of the incubator and inspected for growth, then OD readings were performed. OD readings. I did gram stains for each of the flasks. The TSB sample did show contamination, indicated by the small spheres mixed in with the rods that are more typical of D. aquaticus . The gram stains also showed some discrepancies. D. aquaticus  is typically Gram negative, however, the stains I did showed a mixture of negative and positive, sometimes within the same sample. LB R2B TGY TSB TSYE With Stacy's assistance, we did PEMS on my samples to be analyzed with MA...
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Week of 9/30/19

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10/1 Today, Chad and I went through our box of DNA isolations and PCR products. We've been working on isolating and running PCR on each of the Deinococcus  species we have so that we can have 16s rRNA sequencing done on them. We decided that since we've had these for some time now, that Chad would run new PCR for the isolations that we already have. Then we'll only need the last two species that we currently have to be grown up, DNA isolated, and PCR run. 10/3 Today I had to make more R2B broth media, and once it was autoclaved, I inoculated from plate to flasks and placed them in the incubator. 10/4 Today, I did some planning and prep for Saturday's inoculations. John and I also mapped out an idea for a growth media database. 10/5 Today, the flasks were removed from the incubator and inspected for growth. All six showed growth, and four of the six showed biofilm formation on the sides of the flasks. Then, John an...
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December 2020

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 This Is It! This is officially the last day of the semester. I made it! Not gonna lie, there was some doubt there for a little while. I am so ready for the break before the next semester starts. I'm planning to catch up on sleep, reading, and household chores. But let's be honest, it's mostly about the sleep.  I hope my STEM-TRAIN colleagues have a nice decompression break, and I look forward to seeing them again next semester.
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