Catching Up!

Saturday, March 24, 2018

First day in the research lab!

When I got in, the group was working on a gel electrophoresis of D. radiodurans bacteria.


It was very cool to actually see the dyed DNA fragments moving through the gel once current was applied.



Friday, March 30, 2018

On Friday, Mel went over how to use the micropipettes, and we spent some time practicing with water Eppendorf tubes. She also went over aseptic procedures associated with using the micropipettes to avoid contamination. 

Saturday, March 31, 2018

On Saturday, we did a transformation! We introduced pGLO plasmid to E. coli HB101 competent cells. The pGLO plasmid contains Green Fluorescent Protein (GFP) which gives cells a green glow when the protein is produced. 

We plated our new, modified bacteria and put the plates in the incubator to grow.
Agar plates labeled and ready to go!


Comments

  1. James GutierrezApril 3, 2019 at 11:35 AM

    I remember doing Electrophoresis in high school, it was pretty fun. We got to mess around with it and we had to solve a crime scene with the gel. We used that machine fairly often because the class was based on DNA sequencing. I had a ton of fun doing that.

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