January 2019

January 2, 2019

Inoculation of Deinococcus bacteria:
  • D. radiodurans
  • D. daejeonensis
  • D. ficus
  • D. pimensis
  • D. hopiensis
  • D. apachensis
  • D. aquaticus (German) 

January 9, 2019

Inoculation of M-Series bacteria. The M-Series is a collection of samples taken from Fox River near Oshkosh, Wisconsin.
  • 47
  • 56
  • 72
  • 124
  • 145
  • 176
  • 267
  • 668
  • 722
  • 754
  • 756
  • 807 (plate to broth inoculation)
  • 914 (plate to broth inoculation)
We also received a new Deinococcus species, D. metalli!
  • appears pink on TGY plate
  • has sedimentary-like colonies in TGY broth
The plate and flask we received from Rhonda were placed back into the incubator for more grow time. 


D. metalli grown on a TGY plate.

D. metalli grown in TGY broth. Note the sedimentary nature of the growth.

January 11, 2019

  • Did a freezeback of the new D. metalli so that we have an original source in the freezer.
  • Aliquoted TGY broth to flasks for inoculation.
  • Made 500 mL of TGY with agar for plates and 250 mL of TGY broth.
Gram stains!

D. apachensis (1)
D. apachensis (2)
D. aquaticus 
D. metalli
D. pimensis

January 15, 2019

Lots and lots of gram stains!
  • M56 - gram positive, rods in chains
  • M72 - gram positive, rods in long chains (look like threads)
  • M88 - gram negative, short rods in long chains
  • M124 - gram positive, large rods in short chains
  • M176 - gram positive, small rods in short chains
  • M184 - gram positive, large rods with "heads" in short chains
  • M591 - gram positive, various sized rods in short chains
  • M654 - gram positive, short rods in chains
  • M825 - gram positive, rods alone or in very short chains
  • D. metalli - gram positive, small rods alone or in pairs, clumped together

M56
M72
M88
M124
M176
M184
M591
M654
    M825
D. metalli





















January 18, 2019


More gram stains!
  • D. apachensis
  • D. aquatilis
  • D. daejeonensis
  • D. metalli
  • D. pimensis
  • D. radiophilus
  • D. roseus
  • M807

D. apachensis
D. aquatilis
D. daejeonensis
D. metalli
D. pimensis
D. radiophilus
D. roseus
M807

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